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Substrate solution is then added and color develops in proportion to the amount of Mouse PTGDS
Substrate solution is then added and color develops in proportion to the amount of Rat CNTN1
The concentration of Rat LDLR in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse ST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
PGC present in the sample is added and binds to antibodies coated on the wells
Human SPRR2B (Small Proline-rich Protein 2B) ELISA Kit SCYA27 Substrate solution is then addedThe Human (SPRR2B) Small Proline rich Protein 2B ELISA Kit measures Small proline rich protein 2B in samples. The plate has been pre coated with Human SPRR2B antibody. SPRR2B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPRR2B Antibody is added and binds to SPRR2B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPRR2B antibody. After incubation unbound Streptavidin
